theoretical investigation of the potential role of the gene sequence provided in adult skeletal muscle stem cell behaviour”
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provided cDNA sequence: TCTAGGTCGACGGTNTCGATAAGCTTGATATCGAATTCCTGCAGCCCGGGGGATCTCTATATGTTTTGATGGGGCTGCACATCATCTTGTCAGGTCCTGTCATTGAAAAGATGGCTCTCAGCTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGTTCTCGCTCGGATGAGCTTACCCGCCATATCCGCATCCACACAGGCCAGAAGCCCTTCCAGTGTCGAATCTGCATGCGTAACTTCAGTCGTAGTGACCACCTTACCACCCACATCCGCACCCACACNGGCGAGAAGCCTTTTGCCTGTGACATTTGTGGGAGGAAGTTTGCCAGGANTGATGANNGNAAGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTCGCGCAGCGTTGACCNNTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTTCGCNTTTCTTCCCTTCCTTTCTCGNCACGT
- Describes the aim of the project and summarizes the essence of the underlying hypothesis
- Describes strategy and methodology
- Describes relevant techniques and skillsdeveloped.
- Describes results and conclusion clearly
- Makes a connection of developed skill
- What is the sequence?
NB. Sometimes, the sequence contains more than one fragment that ligated together during the cloning procedure prior to sequencing
– Is the sequence ALL one transcript?
– Is there any genomic sequence?
- What is the structure of the transcript (introns/exons)
Is there more than one splice variant?
Is it part of a family?
- What is the protein product?
– Are there any functional domains?
– What do these domains do?
- What pathway(s)/functions does this gene product play a role in?
- What is known about the expression patterns of the gene (during development and in the adult)